COMPARISON OF ADHESIVE PROPERTIES OF STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI STRAINS ISOLATED FROM THE VAGINAS OF MICE IN NORMAL AND WITH DYSBIOSIS
DOI:
https://doi.org/10.32782/1998-6475.2025.58.2Keywords:
staphylococci, Escherichia coli, adhesion, film formation, microbial biofilmsAbstract
The work investigated 34 strains of Staphylococcus aureus and 24 strains of Escherichia coli isolated from the reproductive tract of healthy mice and mice with vaginal dysbiosis. From animals without signs of pathology, 9 strains of S. aureus and 1 strain identified as E. coli were isolated. From animals with vaginal dysbiosis induced by intravaginal administration of a film-forming strain of S. aureus, 14 strains of S. aureus and 12 strains of E. coli were isolated. From animals with vaginal dysbiosis induced by intravaginal administration of a non-film-forming strain of S. aureus, 11 strains of S. aureus and 11 strains of E. coli were isolated. This indicates a greater species diversity of opportunistic microorganisms under conditions of dysbiosis. The increase in the number of representatives of 1 strain of S. aureus as a result of the induction of dysbiosis led to the facilitation of colonization of the biotope by other strains of this species and even other types of bacteria, in particular E. coli.It was determined that more pronounced adhesive properties were possessed by strains isolated in dysbiosis caused by intravaginal administration of a film-forming strain of S. aureus. Thus, among staphylococci, strains with average adhesiveness indices (50.0 %) prevailed, 35.7 % of strains were highly adhesive, 14.3 % were low-adhesive. The average adhesion index ranged from 1.36 ± 0.48 to 4.00 ± 0.78. The erythrocyte participation factor was 79.64 ± 2.65 %, and the adhesion index of microorganisms varied from 1.81 to 5.19. Among Escherichia coli strains, 75.0 % were highly adhesive, and 25.5 % were medium-adhesive. Average adhesion index ranged from 3.02 ± 0.74 to 4.96 ± 0.78.Erythrocyte participation factor was 84.00 ± 2.95 %, and adhesion index of microorganisms ranged from 3.55 to 6.12.This indicates their higher pathogenicity potential due to increased colonization ability of vaginal mucosal surfaces.
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