MICROCLONAL PROPAGATION OF THE SPECIES FROM THE COLLECTION OF THE BOTANICAL GARDEN: ACER PLATANOIDES L. “CRIMSON KING” (ACERACEAE JUSS.)
DOI:
https://doi.org/10.32782/1998-6475.2025.58.22Keywords:
Acer platanoides L., in vitro, arid climatic conditions, steppe zone, microclonal cultureAbstract
Acer platanoides L. is widely used in ornamental landscaping throughout the steppe zone of Ukraine and in Uzbekistan with a temperate continental climate. Due to its striking dark purple foliage, it creates a striking contrast in gardens, parks and urban green spaces. As a shade tree, it provides aesthetic appeal and excellent canopy cover, where it affects cooling, regulating the microclimate, making it a popular choice for streets, residential yards and public spaces. Its resistance to pollution, compacted soil and urban conditions makes it particularly suitable for urban environments, where it is often planted along roads and in public parks. Microclonal propagation of the ornamental species Acer platanoides L. “Crimson King” was achieved through indirect organogenesis and microcutting.Indirect organogenesis was induced on leaf petioles using DKW medium with 2.4D (0.5 mg/l) + Kin (0.5 mg/l) or 2.4D (0.5 mg/l) + BAP (0.5 mg/l), followed by subculturing onto BAP-supplemented medium (0.01–1.0 mg/l).Microcutting was performed on hormone-free WPM medium with 20 % sucrose, then subcultured onto BAP (0.01–1.0 mg/l) before transferring to a medium containing NAA (2 mg/l) + BAP (0.5 mg/l) + TDZ (0.5 mg/l).
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